| 李梦云,张华,刘亚丽,张慧,庄雪晴.四环素和铜离子对生物除磷中微生物胞外聚合物的影响[J].安徽建筑大学学报,2022,30(4):66-71，91 |
| 四环素和铜离子对生物除磷中微生物胞外聚合物的影响 |
| Effects of Tetracycline and Copper Ion on Extracellular Polymeric Substances During Biological Phosphorus Remova |
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| DOI： |
| 中文关键词: 四环素 铜离子 胞外聚合物 生物除磷 |
| 英文关键词: tetracycline copper ion extracellular polymeric substances biological phosphorus removal |
| 基金项目:安徽省高校省级自然科学研究项目（KJ2019ZD52，KJ2020A0466）；安徽省自然科学基金面上项目（1908085ME142， 1808085MB34）；安徽建筑大学引进人才及博士启动基金项目（2019QDZ64，2019QDZ60）；国家重点研发计划专项项目 （2019YFC0408504） |
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| 中文摘要: |
| 微生物胞外聚合物是保护细胞抵御外界有毒有害物质的“天然保护膜”，而胞外聚合物会受到各种污染物质的影响。选取四环素和铜离子作为污染物，研究其对污水生物除磷中微生物胞外聚合物中蛋白质和多糖的影响。结果表明，四环素作用36h时，蛋白质和多糖含量最大分别为78.39mg/gVSS和15.48mg/gVSS；铜离子作用48h时，蛋白质和多糖含量最大分别为75.93mg/gVSS和14.12mg/gVSS。三种混合物浓度配比分别为0.297、0.894和2.676时，36h时蛋白质含量最大分别为75.60mg/gVSS、68.81mg/gVSS和70.02mg/gVSS，多糖含量最大分别为12.77mg/gVSS、12.10mg/gVSS和13.49mg/gVSS。作用时间增长，胞外聚合物出现先增加后减小的现象，主要因为污染物投加初期微生物分泌更多聚合物抵抗不利环境，而后期由于微生物失活最终导致微生物胞外聚合物减少。混合物浓度增加，微生物胞外聚合物中蛋白质三维荧光强度逐渐减弱。该研究对揭示污染物对生物除磷微生物的影响具有一定的意义。 |
| 英文摘要: |
| Extracellular polymers of microorganisms are natural protective membranes that protect cells against external toxic andharmful substances，but can be affected by various contaminants. Tetracycline and copper ions were selected as contaminants to studytheir effects on protein and polysaccharides in microbial extracellular polymers during biological phosphorus removal. The resultsshowed that the maximum contents of protein and polysaccharide were 78.39 mg/gVSS and 15.48 mg/gVSS，respectively，at 36 h withtetracycline，and 75.93 mg/gVSS and 14. 12 mg/gVSS，respectively，at 48 h with copper ions. Three concentration ratios（0.297，0.894and 2.676） were formulated using a mixture of tetracycline and copper ions，and at 36 h，the maximum protein content was 75.60mg/gVSS，68.81 mg/gVSS and 70.02 mg/gVSS，respectively，while the maximum polysaccharide content was 12.77 mg/gVSS，12.10 mg/gVSS and 13.49 mg/gVSS，respectively. The extracellular polymer increases and then decreases as the time of action increases，mainly because the microorganisms secrete more polymers to resist contaminants at the beginning，while microbial deactivation later ledto a decrease in microbial extracellular polymers. The three-dimensional fluorescence intensity of proteins in the microbial extracellularpolymer gradually decreased as the concentration of the mixture increasing. This study has implications for studying impacts of pollutantson biological phosphorus removal microorganisms. |
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