| 罗月颖,冯青原,杨英.MAS-SBR系统中耐盐菌筛选及耐盐机理研究[J].安徽建筑大学学报,2026,34(3):74-80 |
| MAS-SBR系统中耐盐菌筛选及耐盐机理研究 |
| Screening of Salt-Tolerant Bacteria and Study on Their Salt-Tolerance Mechanisms in the MAS-SBR System |
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| DOI： |
| 中文关键词: 含盐废水 磁性活性污泥 胞外聚合物 微生物多样性 耐盐菌 耐盐机理 |
| 英文关键词: saline wastewater magnetic activated sludge extracellular polymer microbial diversity halotolerant bacteria salttolerance mechanism |
| 基金项目:安徽省重大科技专项项目（202003a06020034） |
| 作者 | 单位 | | 罗月颖 | School of Urban Construction,Anhui Xinhua University,Hefei 230088,China | | 冯青原 | School of Environment and Energy Engineering,Anhui Jianzhu University,Hefei 230601,China | | 杨英 | School of Urban Construction,Anhui Xinhua University,Hefei 230088,China；School of Environment and Energy Engineering,Anhui Jianzhu University,Hefei 230601,China |
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| 中文摘要: |
| 本文以磁活性污泥-序批式反应器（MAS-SBR）体系为研究对象，开展耐盐功能菌筛选及其耐盐作用机理研究。从盐度40 g/L的磁性活性污泥中分离得到菌株B1、B2，菌种鉴定结果表明B1为橄榄芽孢杆菌，B2为盐枝芽孢杆菌；在盐度0 ~ 90 g/L区间内两株菌均可正常生长，其中B1对高盐环境的适应性更优；B1最适生长pH为8，B2最适生长pH为7。当环境盐度超出菌株耐受阈值时，耐盐菌B1、B2可协同调控胞内渗透压稳态：一方面开启阴阳离子转运通道（如SO42-、Ca2+、NO3-和K+），摄取脯氨酸、甘氨酸、海藻糖、甜菜碱等相容性溶质；另一方面下调胞内蛋白质合成、提升多糖消耗水平，通过多重途径共同维持细胞渗透压平衡。 |
| 英文摘要: |
| This study mainly focuses on the screening of salt-tolerant bacteria and the research on salt-tolerance mechanisms in the MAS-SBR system. Strains B1 and B2 were isolated from magnetic activated sludge with a salinity of 40 g/L. Through identification, B1 was identified as Bacillus olivaceus and B2 as Bacillus saliphilus. Both salt-tolerant strains B1 and B2 can grow within the salinity range of 0–90 g/L, but strain B1 shows better adaptability to high-salt environments. The optimal pH value for strain B1 is 8, while that for B2 is 7. When salt-tolerant strains B1 and B2 are in a high-salt environment exceeding their tolerance limit, they can cooperate in osmotic homeostasis by operating cation and anion channels（such as SO4 2- 、Ca2+ 、NO3 - and K+ ）, absorbing compatible substances （proline, glycine, trehalose, and betaine）, reducing intracellular protein synthesis, and increasing polysaccharide consumption. |
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