| 李丽,张华,张文超.不同碳源调控辅酶I对聚磷菌胞内多聚物代谢的影响[J].安徽建筑大学学报,2021,29(1):68-72 |
| 不同碳源调控辅酶I对聚磷菌胞内多聚物代谢的影响 |
| Effects of Coenzyme Ⅰ on Intracellular Polymer Metabolism of Phosphate Accumulating Organisms |
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| DOI： |
| 中文关键词: 碳源 辅酶 Ι PHB poly-P 糖原 |
| 英文关键词: carbon source coenzyme I PHB poly-P glycogen |
| 基金项目: |
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| 摘要点击次数: 6211 |
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| 中文摘要: |
| 辅酶 Ι 是聚磷菌胞内物质代谢的关键酶，研究不同碳源调控辅酶 Ι 对聚磷菌胞内 PHB、poly-P、糖
原代谢的影响对强化生物除磷具有重要的意义。研究结果表明，以乙酸钠为碳源对胞内辅酶 Ι 进行调控时，
胞内 NAD+
（氧化型辅酶 Ι）、NADH（还原型辅酶 Ι）平均含量分别为 3.547 μmol/g、3.255 μmol/g，以葡萄
糖为碳源对胞内辅酶 Ι 进行调控时，胞内 NAD+
、NADH 平均含量分别为 1.592 μmol/g、1.495 μmol/g，说明
以乙酸钠为碳源对胞内辅酶 Ι 的调控效果更好。不同碳源调控胞内辅酶 Ι 对聚磷菌胞内多聚物代谢影响的
研究结果表明，乙酸钠为碳源进行调控辅酶 Ι 时，胞内 PHB 和 poly-P 的合成量更高，聚磷菌除磷效率更好，
因此，以乙酸钠为碳源调控聚磷菌胞内辅酶 Ι 时有助于提高胞内多聚物的代谢能力。说明通过不同碳源调控
聚磷菌胞内 NADH 的含量可以提高生物除磷的效果，为强化生物除磷效率提供一种新思想。 |
| 英文摘要: |
| During the biological phosphorus removal，coenzyme I is the key enzyme for the intracellular polymer metabolism of
phosphate accumulating organisms. Therefore，it is important to study the effects of coenzyme on the metabolism of PHB，poly-P and
glycogen of phosphate accumulating organisms. In this study，different carbon were used to regulate the coenzyme of the phosphate
accumulating organisms，and then the metabolism of the intracellular polymers was further studied. The results showed that，when
sodium acetate was used as the carbon of the phosphate accumulating organisms，the average contents of NAD+ （oxidized coenzyme
I） and NADH （reduced coenzyme I） were respectively 3.547 μmol/g and 3.255 μmol/g. However，when glucose was used as the
carbon of the phosphate accumulating organisms，the average contents of NAD+ and NADH were 1.592 μmol/g and 1.495 μmol/g
respectively. The research indicated that，when sodium acetate was used as the carbon of the phosphate accumulating organisms，there
was a better regulated effect on coenzyme I. The metabolisms of the intracellular polymers indicated that，when sodium acetate was
used as the carbon to regulate coenzyme I，the synthesis of the intracellular PHB and poly-P were higher. The higher the synthesis of
the intracellular PHB and poly-P were，the better the phosphorus removal efficiency was. Therefore，when sodium acetate was used as
carbon to regulate the intracellular coenzyme I，it was helpful to improve the metabolic capacity of intracellular polymers. The results showed that the biological phosphorus removal may be improved by regulating the intracellular coenzyme I of phosphate accumulating
organism. This study provides a new idea for enhancing biological phosphorus removal. |
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