| 张华,马涛,李丽,黄健.生物除磷工艺中高效聚磷菌的快速筛选和鉴定[J].安徽建筑大学学报,2020,28(1):69-72 |
| 生物除磷工艺中高效聚磷菌的快速筛选和鉴定 |
| Rapid Screening and Identification of Efficient Phosphorus AccumulatingOrganisms in Biological Phosphorus Removal Process |
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| DOI：10.11921/j.issn.2095-8382.20200111 |
| 中文关键词: 生物除磷 高效聚磷菌 筛选和鉴定 |
| 英文关键词: biological phosphorus removal process efficient polyphosphate bacteria screening and identification |
| 基金项目:安徽省自然科学研究面上项目(1808085MB34，1908085ME142)；安徽省高校省级自然科学研究项目重大项目(KJ2019ZD52)。 |
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| 中文摘要: |
| 通过厌氧/好氧交替平板迭代法培养活性污泥，采用5-溴-4-氯-3-吲哚基-磷酸盐(BCIP)蓝白斑筛选法、
PHB 染色、异染颗粒染色进行高效聚磷菌的快速筛选，并基于16 S rDNA 对各菌株进行高通量测序和鉴定。
结果表明，4 株菌可以分解BCIP 产生蓝绿色菌落且厌氧PHB 和好氧异染颗粒染色为蓝黑色，除磷率分别为1
号菌85.51%，2 号菌91.06％，3 号菌95.51%，4 号菌89.13%。高通量测序结果表明，1 号菌为Exiguobacterium
sp.ZWU0009(微小杆菌属)，2 号菌为Bacillus sp. C10(芽孢杆菌属)，3 号菌为Exiguobacteriumacetylicum
strain SI17(乙酰杆菌属)，4 号菌为Exiguobacteriumacetylicum strain BGSLP4(乙酰杆菌属)。 |
| 英文摘要: |
| The activated sludge was cultured by plate iteration method with alternating anaerobic and aerobic operation.
Efficient phosphorus accumulating organisms were rapidly screened using 5-bromo-4-chloro-3-indolyl-phosphate (BCIP)，
dyeing of PHB and heterochromatic particles. Each strain was sequenced and identified by high-throughput sequencing
based on 16S rDNA. The results showed that the four strains could decompose BCIP and produce blue-green. After staining，
the PHB in the anaerobic stage and the heterodye particles in the aerobic stage were all blue black. The phosphorus
removal rates of the four strains were 85.51% (No.1 strain)，91.06% (No.2 strain)，95.51% (No.3 strain)，89.13% (No.4
strain) respectively. The results of high-throughput sequencing showed that the No. 1 strain was Exiguobacterium sp.
ZWU0009，the No.2 strainwas Bacillus sp. C10，the No.3 strainwasExiguobacteriumacetylicum strain SI17，and the No.4
strainwasExiguobacteriumacetylicum strain BGSLP4. |
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