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| MAS-SBR系统中耐盐菌筛选及耐盐机理研究 |
| Screening of Salt-Tolerant Bacteria and Study on Their Salt-Tolerance Mechanisms in the MAS-SBR System |
| 投稿时间：2025-11-14 修订日期：2026-03-13 |
| DOI： |
| 中文关键词: 含盐废水 磁性活性污泥 胞外聚合物 微生物多样性 耐盐菌 耐盐机理 |
| 英文关键词: Saline wastewater Magnetic activated sludge Extracellular polymer Microbial diversity Halotolerant bacteria Salt tolerance mechanism. |
| 基金项目:安徽省重大科技专项(202003a06020034) |
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| 中文摘要: |
| 本文主要对磁活性污泥-序批式反应器（MAS-SBR）系统中耐盐菌筛选及耐盐机理进行研究。从盐度为40 g/L的磁性活性污泥中筛选出菌株B1和 B2，经鉴定 B1为橄榄芽孢杆菌，B2为盐枝芽孢杆菌；在0～90 g/L 盐度浓度范围内，菌株 B1和 B2都能生长，但B1能更好的适应高盐环境；B1菌株最适 pH 值为 8，B2菌株最适pH值为7。当耐盐菌株B1和B2在超过自身所承受的高盐环境中时，可以通过运转阴阳离子通道（如 SO4 2-、Ca 2+、 NO3 - 和 K+ ）和吸收相容性物质（脯氨酸、甘氨酸、海藻糖和甜菜碱）以及降低细胞内蛋白质合成，提高多糖消耗，协同调节渗透压稳态。 |
| 英文摘要: |
| This study mainly focuses on the screening of salt-tolerant bacteria and the research on salt-tolerance mechanisms in the MAS-SBR system. Strains B1 and B2 were isolated from magnetic activated sludge with a salinity of 40 g/L. Through identification, B1 was identified as Bacillus olivaceus and B2 as Bacillus saliphilus. Both salt-tolerant strains B1 and B2 can grow within the salinity range of 0–90 g/L, but strain B1 shows better adaptability to high-salt environments. The optimal pH value for strain B1 is 8, while that for B2 is 7. When salt-tolerant strains B1 and B2 are in a high-salt environment exceeding their tolerance limit, they can cooperate in osmotic homeostasis by operating cation and anion channels (such as SO4 2-、Ca 2+、 NO3 - and K+), absorbing compatible substances (proline, glycine, trehalose, and betaine), reducing intracellular protein synthesis, and increasing polysaccharide consumption. |
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